Secondary antibody dilution western blot9/15/2023 ![]() After protein transfer, wash the membrane in deionized water 4 times for 5 minutes each with agitation to remove all transfer buffer.Follow manufacture instructions for wet, semi-dry, or dry transfer.Nitrocellulose: equilibrate directly in transfer buffer for 5 minutes.PVDF: pre-wet in methanol or ethanol (100%) for 30 seconds, briefly rinse in deionized water, and equilibrate in transfer buffer for 5 minutes.Follow manufacture instructions for dry membrane preparations. ![]()
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